Molecular role of Leucine-rich repeat kinase2 (LRRK2) in Parkinson’s disease models
نویسنده
چکیده
65 2.1 Material and Methods 66 2.1.1 Plasmid and cloning 66 2.1.2 Cell culture and transfection 66 2.1.3 Assessment of cell viability 67 2.1.4 Assessment of Neurite process length and branching point. 68 2.1.5 Ras Pull-Down Assay 68 2.1.6 Generation of Human LRRK2 Transgenic Flies. 69 2.1.7 Western Blot Analysis. 70 2.1.8 Immunoprecipitation. 71 2.1.9 Optical Neutralization Technique. 71 2.1.10 Cryosections 72 2.1.11 Survival Assay. 72 2.1.12 Climbing Assay. 72 2.1.13 Immunostaining and quantification of dopaminergic neurons. 72 2.1.14 Data Analysis. 73
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تاریخ انتشار 2010